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NRF2 is a master regulator of antioxidant response as a transcription factor regulating expression of several enzymes responsible for glutathione synthesis, including glutamate-cysteine ligase modifier subunit (GCLM) and the glutamate-cysteine catalytic subunit (GCLC), as well as enzymes related to GSH utilization, such as glutathione reductase, glutathione peroxidase and glutathione S transferase (GST) [45,46]

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After washing, the pellet was dissolved in 800 μl of 9 M urea in 0.4 N NaOH, then incubated at 37 °C for 10 min