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Patients infected with SARS-CoV-2 exhibit alterations in the glucose–insulin axis, including increased mitochondrial damage, oxidative/nitrosative stress, and significantly reduced levels of vitamin D, thiols, total antioxidant capacity (TAC), GSH, and selenium, resulting in hyperglycemia, hyperinsulinemia, and insulin resistance [153,154]

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Two of the 6 clones, Clones #5 and #9, were identified as having mutations at the genetic lesion targeted using GLA-specific sgRNA-mediated CRISPR/Cas9 and were used for western blot analysis