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Talon pull-down assay The bait, MTRR FL with a N-terminal His-tag at 0.5 mg ml −1 was mixed with either prey proteins MTR FL , MTR N-Half or MTR C-Half without His-tags at 0.5 mg ml −1 in a total volume of 100 μl diluted in binding buffer (25 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM TCEP, 0.2% Tween-20)