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This discovery led us to postulate that cross-link formation was responsible for the commonly observed migration of CDO as two bands on sodium dodecyl sulfate polyacryl-amide gel electrophoresis (SDS-PAGE), and we verified that formation of this cross-link resulted in slightly faster migration of CDO on SDS-PAGE, with an apparent molecular mass of 22.5 kDa instead of the 23-kDa mass of immature CDO without the Cys-Tyr cross-link (Dominy et al
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